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KMID : 0545120150250101640
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 10 p.1640 ~ p.1647
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Antimicrobial Peptide, Lumbricusin, Ameliorates Motor Dysfunction and Dopaminergic Neurodegeneration in a Mouse Model of Parkinson’s Disease
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Kim Dae-Hong
Lee Ik-Hwan
Nam Seung-Taek
Hong Ji
Zhang Peng
Lu Li Fang
Hwang Jae-Sam
Park Ki-Cheol
Kim Ho
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Abstract
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We recently reported that the antimicrobial peptide Lumbricusin (NH2-RNRRWCIDQQA), isolated from the earthworm, increases cell proliferation in neuroblastoma SH-SY5Y cells. Here, we investigated whether Lumbricusin has neurotropic activity in mouse neural stem cells (MNSCs) and a protective effect in a mouse model of Parkinson’s disease (PD). In MNSCs isolated from mouse brains, Lumbricusin treatment significantly increased cell proliferation (up to 12%) and reduced the protein expression of p27Kip1 through proteasomal protein degradation but not transcriptional regulation. Lumbricusin inhibited the 6-OHDA-induced apoptosis of MNSCs, and also showed neuroprotective effects in a mouse PD model, ameliorating the motor impairments seen in the pole, elevated body swing, and rotation tests. These results suggest that the Lumbricusin-induced promotion of neural cell proliferation via p27Kip1 degradation has a protective effect in an experimental PD model. Thus, the antimicrobial peptide Lumbricusin could possibly be developed as a potential therapeutic agent for the treatment of PD.
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KEYWORD
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Antimicrobial peptide, Parkinson’s disease, neuroprotective activity, cell proliferation (neurotropine), proteasomal protein degradation, cell cycle arrest
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